Plasmid
pJEJAM5

Part:BBa_K678053:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-13)

pJEJAM5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2207
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
    Illegal NgoMIV site found at 3062
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This plasmid was assembled with the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this plasmid contains linkers between the biological parts as shown on the figure below.


DTU-Denmark-2 2011



Source

See the individual biobricks

References